A mass chromatogram is a representation of mass spectrometry data as a chromatogram , where the x-axis represents time and the y-axis represents signal intensity. The source data contains mass information; however, it is not graphically represented in a mass chromatogram in favor of visualizing signal intensity versus time. The most common use of this data representation is when mass spectrometry is used in conjunction with some form of chromatography , such as in liquid chromatography–mass spectrometry or gas chromatography–mass spectrometry . In this case, the x-axis represents retention time, analogous to any other chromatogram. The y-axis represents signal intensity or relative signal intensity. There are many different types of metrics that this intensity may represent, depending on what information is extracted from each mass spectrum.
22-645: [REDACTED] Look up EIC in Wiktionary, the free dictionary. EIC may refer to: Companies and organizations [ edit ] East India Company , a major British company that once controlled major parts of the Indian subcontinent East India Club , a gentlemen's club in London East India Comedy , an Indian comedy group Edison Illuminating Company , an electric power company in
44-464: A SIM experiment; for extracted-ion chromatograms (EIC or XIC), data for an entire mass range are collected during the run and then examined for analytes of interest after the completion of the run. The selected-reaction monitoring (SRM) experiment is very similar to the SIM experiment except that tandem mass spectrometry is used and a specific product ion of a specific parent ion is detected. The mass of
66-837: A gentlemen's club in London East India Comedy , an Indian comedy group Edison Illuminating Company , an electric power company in the United States Emmanuel International Canada , a non-profit, evangelical Christian relief organization Encompass Insurance Company , of New Jersey Energy in Common , an American renewable energy organization Engineering Institute of Canada Ensemble InterContemporain , an orchestra in Paris Entertainment Industries Council , an American public health organization Environmental Information Coalition , creators of
88-792: A journalism organization Schools [ edit ] Ealing Independent College , in West London Edinburgh International College , in Scotland Cherbourg School of Engineering (French: École d'Ingénieurs de Cherbourg ), in France International School of Carthage (French: École Internationale de Carthage ), in Tunis, Tunisia Other [ edit ] Education in Chemistry ,
110-420: A journalism organization Schools [ edit ] Ealing Independent College , in West London Edinburgh International College , in Scotland Cherbourg School of Engineering (French: École d'Ingénieurs de Cherbourg ), in France International School of Carthage (French: École Internationale de Carthage ), in Tunis, Tunisia Other [ edit ] Education in Chemistry ,
132-534: A magazine Earned Income Credit , in the United States East Iceland Current Eastern Indiana Conference , in northeastern Indiana from 1953 to 1975 Eastern Intercollegiate Conference , a defunct NCAA conference Editor-in-chief Electron–ion collider , a proposed type of particle accelerator collider Electronic identity card Employer Identification Number , assigned by
154-407: A magazine Earned Income Credit , in the United States East Iceland Current Eastern Indiana Conference , in northeastern Indiana from 1953 to 1975 Eastern Intercollegiate Conference , a defunct NCAA conference Editor-in-chief Electron–ion collider , a proposed type of particle accelerator collider Electronic identity card Employer Identification Number , assigned by
176-471: A particular analyte's mass-to-charge ratio is plotted at every point in the analysis. The size of the mass tolerance window typically depends on the mass accuracy and mass resolution of the instrument collecting the data. This is useful for re-examining data to detect previously-unsuspected analytes, to highlight potential isomers, resolve suspected co-eluting substances, or to provide clean chromatograms of compounds of interest. An extracted-ion chromatogram
198-431: Is different from Wikidata All article disambiguation pages All disambiguation pages EIC [REDACTED] Look up EIC in Wiktionary, the free dictionary. EIC may refer to: Companies and organizations [ edit ] East India Company , a major British company that once controlled major parts of the Indian subcontinent East India Club ,
220-410: Is different from Wikidata All article disambiguation pages All disambiguation pages Mass chromatogram#Extracted-ion chromatogram (XIC, EIC) The total ion current chromatogram (TICC) represents the summed intensity across the entire range of masses being detected at every point in the analysis. The range is typically several hundred mass-to-charge units or more. In complex samples,
242-413: Is generated by separating the ions of interest from a data file containing the full mass spectrum over time after the fact; this is different from selected-ion chromatograms, discussed below, in which data is collected only for specific m/z values. A closely related term is extracted-compound chromatogram (ECC). A selected-ion monitoring (SIM) chromatogram is similar to an EIC/XIC, with the exception that
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#1732766069366264-741: The Encyclopedia of Earth Estonian Iraqi Contingent , unit of the Estonian military in Iraq European Innovation Council , introduced by the European Commission to support the commercialization of high-risk, high-impact ideas European International Contractors , association, affiliated by its members of European international contractors, dealing with all questions arising from international construction European Investigative Collaborations ,
286-468: The Encyclopedia of Earth Estonian Iraqi Contingent , unit of the Estonian military in Iraq European Innovation Council , introduced by the European Commission to support the commercialization of high-risk, high-impact ideas European International Contractors , association, affiliated by its members of European international contractors, dealing with all questions arising from international construction European Investigative Collaborations ,
308-523: The TICC often provides limited information as multiple analytes elute simultaneously, obscuring individual species. The base peak chromatogram is similar to the TICC, however it monitors only the most intense peak in each spectrum. This means that the base peak chromatogram represents the intensity of the most intense peak at every point in the analysis. Base peak chromatograms often have a cleaner look and thus are more informative than TIC chromatograms because
330-446: The United States Emmanuel International Canada , a non-profit, evangelical Christian relief organization Encompass Insurance Company , of New Jersey Energy in Common , an American renewable energy organization Engineering Institute of Canada Ensemble InterContemporain , an orchestra in Paris Entertainment Industries Council , an American public health organization Environmental Information Coalition , creators of
352-722: The United States Internal Revenue Service Energy Identification Code , to identify gas and electricity assets, actors, and jurisdictions in Europe Excellence in Cities , a British government program Exercise-induced collapse , a genetic syndrome in dogs Extracted-ion chromatogram , a representation of data in hyphenated mass spectrometry/chromatography See also [ edit ] East India Company (disambiguation) Topics referred to by
374-469: The United States Internal Revenue Service Energy Identification Code , to identify gas and electricity assets, actors, and jurisdictions in Europe Excellence in Cities , a British government program Exercise-induced collapse , a genetic syndrome in dogs Extracted-ion chromatogram , a representation of data in hyphenated mass spectrometry/chromatography See also [ edit ] East India Company (disambiguation) Topics referred to by
396-405: The background is reduced by focusing on a single analyte at every point. In an extracted-ion chromatogram (EIC or XIC), also called a reconstructed-ion chromatogram (RIC), one or more m/z values representing one or more analytes of interest are recovered ('extracted') from the entire data set for a chromatographic run. The total intensity or base peak intensity within a mass tolerance window around
418-409: The mass spectrometer is operated in SIM mode, such that only preselected m/z values are detected in the analysis. SIM experiments can be performed using mass spectrometry (MS) or tandem mass spectrometry (MS/MS) instruments. They are more common on MS instruments. This differs significantly from the extracted-ion chromatogram mentioned above in that only data for the ion(s) of interest are collected in
440-511: The parent analyte is first selected while other ions are filtered away. The parent analyte ion is then fragmented in the gas phase and a specific fragment ion is monitored. This experiment has very high specificity because the SRM chromatogram represents only ions of a particular mass that fragment in a manner that produce a very specific product mass. This type of experiment can only be performed using tandem mass spectrometry. The technology progress in
462-449: The same term [REDACTED] This disambiguation page lists articles associated with the title EIC . If an internal link led you here, you may wish to change the link to point directly to the intended article. Retrieved from " https://en.wikipedia.org/w/index.php?title=EIC&oldid=1240234517 " Category : Disambiguation pages Hidden categories: Articles containing French-language text Short description
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#1732766069366484-449: The same term [REDACTED] This disambiguation page lists articles associated with the title EIC . If an internal link led you here, you may wish to change the link to point directly to the intended article. Retrieved from " https://en.wikipedia.org/w/index.php?title=EIC&oldid=1240234517 " Category : Disambiguation pages Hidden categories: Articles containing French-language text Short description
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