Pfu DNA polymerase is an enzyme found in the hyperthermophilic archaeon Pyrococcus furiosus , where it functions to copy the organism's DNA during cell division ( thermostable DNA polymerase ). In the laboratory setting, Pfu is used to amplify DNA in the polymerase chain reaction (PCR), where the enzyme serves the central function of copying a new strand of DNA during each extension step.
5-560: [REDACTED] Look up pfu in Wiktionary, the free dictionary. PFU may refer to: PFU Limited , a Japanese information technology company Plaque-forming unit , a measure used in virology Peoples' Friendship University of Russia , an educational and research institution located in Moscownonaligned countries Pfu DNA polymerase , an enzyme Topics referred to by
10-501: Is different from Wikidata All article disambiguation pages All disambiguation pages pfu It is a family B DNA polymerase . It has an RNase H -like 3'-5' exonuclease domain, typical of B-family polymerase such as DNA polymerase II . Pfu DNA polymerase has superior thermostability and proofreading properties compared with Taq DNA polymerase . Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity, meaning that as
15-453: Is slower and typically requires 1–2 minutes per cycle to amplify 1kb of DNA at 72 °C. Using Pfu DNA polymerase in PCR reactions also results in blunt-ended PCR products. Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with
20-522: The DNA is assembled from the 5' end to 3' end , the exonuclease activity immediately removes nucleotides misincorporated at the 3' end of the growing DNA strand. Consequently, Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq -generated PCR inserts. Commercially available Pfu typically results in an error rate of 1 in 1.3 million base pairs and can yield 2.6% mutated products when amplifying 1 kb fragments using PCR. However, Pfu
25-402: The same term [REDACTED] This disambiguation page lists articles associated with the title PFU . If an internal link led you here, you may wish to change the link to point directly to the intended article. Retrieved from " https://en.wikipedia.org/w/index.php?title=PFU&oldid=993528561 " Category : Disambiguation pages Hidden categories: Short description
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