21-3190: 1A2C , 1A3B , 1A3E , 1ABI , 1ABJ , 1AD8 , 1AE8 , 1AI8 , 1AIX , 1AWF , 1AWH , 1AY6 , 1B5G , 1B7X , 1BA8 , 1BB0 , 1BCU , 1BHX , 1BMM , 1BMN , 1BTH , 1C1U , 1C1V , 1C1W , 1C4U , 1C4V , 1C4Y , 1C5L , 1C5N , 1C5O , 1CA8 , 1D3D , 1D3P , 1D3Q , 1D3T , 1D4P , 1D6W , 1D9I , 1DE7 , 1DIT , 1DM4 , 1DOJ , 1DWB , 1DWC , 1DWD , 1DX5 , 1E0F , 1EB1 , 1EOJ , 1EOL , 1FPC , 1G30 , 1G32 , 1G37 , 1GHV , 1GHW , 1GHX , 1GHY , 1GJ4 , 1GJ5 , 1H8D , 1H8I , 1HAI , 1HAO , 1HAP , 1HBT , 1HLT , 1HUT , 1HXE , 1HXF , 1IHS , 1JMO , 1JOU , 1JWT , 1K21 , 1K22 , 1KTS , 1KTT , 1LHC , 1LHD , 1LHE , 1LHF , 1LHG , 1MH0 , 1MU6 , 1MU8 , 1MUE , 1NM6 , 1NRN , 1NRO , 1NRP , 1NRQ , 1NRR , 1NRS , 1NT1 , 1NU7 , 1NU9 , 1NY2 , 1NZQ , 1O0D , 1O2G , 1O5G , 1OOK , 1OYT , 1P8V , 1PPB , 1QBV , 1QHR , 1QJ1 , 1QJ6 , 1QJ7 , 1QUR , 1RD3 , 1RIW , 1SB1 , 1SFQ , 1SG8 , 1SGI , 1SHH , 1SL3 , 1SR5 , 1T4U , 1T4V , 1TA2 , 1TA6 , 1TB6 , 1THP , 1THR , 1THS , 1TMB , 1TMU , 1TOM , 1TQ0 , 1TQ7 , 1TWX , 1UVS , 1VR1 , 1VZQ , 1W7G , 1WAY , 1WBG , 1XM1 , 1XMN , 1YPE , 1YPG , 1YPJ , 1YPK , 1YPL , 1YPM , 1Z71 , 1Z8I , 1Z8J , 1ZGI , 1ZGV , 1ZRB , 2A0Q , 2A2X , 2A45 , 2AFQ , 2ANK , 2ANM , 2B5T , 2BDY , 2BVR , 2BVS , 2BVX , 2BXT , 2BXU , 2C8Y , 2FEQ , 2FES , 2GDE , 2GP9 , 2H9T , 2HGT , 2HNT , 2HPP , 2HPQ , 2HWL , 2JH0 , 2JH6 , 2OD3 , 2PGB , 2PGQ , 2PW8 , 2R2M , 2THF , 2ZFQ , 2ZFR , 2ZG0 , 2ZHE , 2ZHF , 2ZHW , 2ZI2 , 2ZIQ , 2ZNK , 2ZO3 , 3B23 , 3B9F , 3BEF , 3BEI , 3BF6 , 3BIU , 3BIV , 3BV9 , 3C1K , 3C27 , 3D49 , 3DA9 , 3DD2 , 3DT0 , 3DUX , 3E6P , 3EE0 , 3EQ0 , 3F68 , 3GIC , 3GIS , 3HAT , 3HKJ , 3HTC , 3JZ2 , 3LDX , 3LU9 , 3NXP , 3P17 , 3P6Z , 3P70 , 3PO1 , 3QGN , 3QLP , 3QTO , 3QTV , 3QWC , 3QX5 , 3R3G , 3RLW , 3RLY , 3RM0 , 3RM2 , 3RML , 3RMM , 3RMN , 3RMO , 3S7H , 3S7K , 3SHA , 3SHC , 3SI3 , 3SI4 , 3SQE , 3SQH , 3SV2 , 3T5F , 3TU7 , 3U69 , 3U8O , 3U8R , 3U8T , 3U98 , 3U9A , 3UTU , 3UWJ , 3VXE , 3VXF , 4BAH , 4BAK , 4BAM , 4BAN , 4BAO , 4BAQ , 4BOH , 4DIH , 4DII , 4DT7 , 4DY7 , 4E05 , 4E06 , 4E7R , 4H6S , 4H6T , 4HFP , 4HTC , 4THN , 5GDS , 7KME , 8KME , 1A46 , 1A4W , 1A5G , 1A61 , 1AFE , 1AHT , 1DWE , 1FPH , 1HAG , 1HAH , 1HDT , 1HGT , 1IHT , 1NO9 , 1TBZ , 1TMT , 1UMA , 2C8W , 2C8X , 2C8Z , 2C90 , 2C93 , 2CF8 , 2CF9 , 2CN0 , 2JH5 , 2PKS , 2UUF , 2UUJ , 2UUK , 2V3H , 2V3O , 2ZC9 , 2ZDA , 2ZDV , 2ZF0 , 2ZFF , 2ZFP , 2ZGB , 2ZGX , 2ZHQ , 3DHK , 3EGK , 3JZ1 , 3K65 , 3PMH , 3QDZ , 4AX9 , 4AYV , 4AYY , 4AZ2 , 4CH2 , 4CH8 , 4HZH , 4I7Y , 4LOY , 4LXB , 4LZ1 , 4LZ4 , 4MLF , 4N3L , 4NZE , 4NZQ , 4O03 , 4RKJ , 4RKO , 4RN6 , 4YES , 4UD9 , 4UDW , 4UE7 , 4UEH , 5AF9 , 5AFY , 5AFZ , 5AHG , 5CMX , 4UFD , 5EDM , 5E8E , 5EDK , 4UFE , 4UFG , 4UFF , 5A2M , 5JDU 2147 14061 ENSG00000180210 ENSMUSG00000027249 P00734 P19221 NM_000506 NM_001311257 NM_010168 NP_000497 NP_034298 Prothrombin ( coagulation factor II )
42-508: A cofactor leads to cleavage of the Gla and two Kringle domains (forming together a fragment called fragment 1.2) and leave thrombin, consisting solely of the serine protease domain. As is the case for all serine proteases , prothrombin is converted to active thrombin by proteolysis of an internal peptide bond, exposing a new N-terminal Ile-NH3. The historic model of activation of serine proteases involves insertion of this newly formed N-terminus of
63-526: A high degree of specificity. Prothrombin complex concentrate and fresh frozen plasma are prothrombin-rich coagulation factor preparations that can be used to correct deficiencies (usually due to medication) of prothrombin. Indications include intractable bleeding due to warfarin . Manipulation of prothrombin is central to the mode of action of most anticoagulants . Warfarin and related drugs inhibit vitamin K -dependent carboxylation of several coagulation factors, including prothrombin. Heparin increases
84-547: A pronounced pro-inflammatory character, which may influence the onset and progression of atherosclerosis. Acting via its specific cell membrane receptors (protease activated receptors: PAR-1, PAR-3 and PAR-4), which are abundantly expressed in all arterial vessel wall constituents, thrombin has the potential to exert pro-atherogenic actions such as inflammation, leukocyte recruitment into the atherosclerotic plaque, enhanced oxidative stress, migration and proliferation of vascular smooth muscle cells, apoptosis and angiogenesis. Thrombin
105-402: Is a valuable biochemical tool. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is commonly included in linker regions of recombinant fusion protein constructs. Following purification of the fusion protein, thrombin can be used to selectively cleave between the arginine and glycine residues of the cleavage site, effectively removing the purification tag from the protein of interest with
126-421: Is approximately 72,000 Da . The catalytic domain is released from prothrombin fragment 1.2 to create the active enzyme thrombin, which has a molecular weight of 36,000 Da. Structurally, it is a member of the large PA clan of proteases. Prothrombin is composed of four domains; an N-terminal Gla domain , two kringle domains and a C-terminal trypsin -like serine protease domain. Factor Xa with factor V as
147-484: Is crucial in physiological and pathological coagulation. Various rare diseases involving prothrombin have been described (e.g., hypoprothrombinemia ). Anti-prothrombin antibodies in autoimmune disease may be a factor in the formation of the lupus anticoagulant (also known as antiphospholipid syndrome ). Hyperprothrombinemia can be caused by the G20210A mutation. Thrombin, a potent vasoconstrictor and mitogen ,
168-566: Is encoded in the human by the F2-gene. It is proteolytically cleaved during the clotting process by the prothrombinase enzyme complex to form thrombin. Thrombin ( Factor IIa ) ( EC 3.4.21.5 , fibrose, thrombase, thrombofort, topical, thrombin-C, tropostasin, activated blood-coagulation factor II, E thrombin, beta-thrombin, gamma-thrombin) is a serine protease , that converts fibrinogen into strands of insoluble fibrin , as well as catalyzing many other coagulation-related reactions. After
189-400: Is greatly enhanced following the binding of thrombin to thrombomodulin , an integral membrane protein expressed by endothelial cells. Activated protein C inactivates factors Va and VIIIa. Binding of activated protein C to protein S leads to a modest increase in its activity. Thrombin is also inactivated by antithrombin , a serine protease inhibitor . The molecular weight of prothrombin
210-404: Is implicated as a major factor in vasospasm following subarachnoid hemorrhage . Blood from a ruptured cerebral aneurysm clots around a cerebral artery , releasing thrombin. This can induce an acute and prolonged narrowing of the blood vessel, potentially resulting in cerebral ischemia and infarction ( stroke ). Beyond its key role in the dynamic process of thrombus formation, thrombin has
231-461: Is implicated in the physiology of blood clots . Its presence indicates the existence of a clot. In 2013 a system for detecting the presence of thrombin was developed in mice. It combines peptide-coated iron oxide attached to "reporter chemicals". When a peptide binds to a thrombin molecule, the report is released and appears in the urine where it can be detected. Human testing has not been conducted. Due to its high proteolytic specificity, thrombin
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#1732786943277252-478: Is sold under the brand name Fibrimex for use as a binding agent for meat. Both proteins in Fibrimex derives from porcine or bovine blood. According to the manufacturer it can be used to produce new kinds of mixed meats (for example combining beef and fish seamlessly). The manufacturer also states that it can be used to combine whole muscle meat, form and portion these, thus cutting down on production costs without
273-436: The prothrombinase complex. Prothrombin is produced in the liver and is co-translationally modified in a vitamin K -dependent reaction that converts 10-12 glutamic acids in the N terminus of the molecule to gamma-carboxyglutamic acid (Gla). In the presence of calcium, the Gla residues promote the binding of prothrombin to phospholipid bilayers. Deficiency of vitamin K or administration of the anticoagulant warfarin inhibits
294-427: The active conformation of thrombin by inserting this N-terminal region. There are an estimated 30 people in the world that have been diagnosed with the congenital form of Factor II deficiency, which should not be confused with the prothrombin G20210A mutation, which is also called the factor II mutation. Prothrombin G20210A is congenital. Prothrombin G20210A is not usually accompanied by other factor mutations (i.e.,
315-549: The affinity of antithrombin to thrombin (as well as factor Xa ). The direct thrombin inhibitors , a newer class of medication, directly inhibit thrombin by binding to its active site. Recombinant thrombin is available as a powder for reconstitution into aqueous solution . It can be applied topically during surgery, as an aid to hemostasis . It can be useful for controlling minor bleeding from capillaries and small venules, but ineffective and not indicated for massive or brisk arterial bleeding. Thrombin, combined with fibrinogen ,
336-503: The blood coagulation pathway, thrombin acts to convert factor XI to XIa, VIII to VIIIa, V to Va, fibrinogen to fibrin , and XIII to XIIIa. In the conversion of fibrinogen into fibrin, thrombin catalyzes the cleavage of fibrinopeptides A and B from the respective Aα and Bβ chains of fibrinogen to form fibrin monomers. Factor XIIIa is a transglutaminase that catalyzes the formation of covalent bonds between lysine and glutamine residues in fibrin. The covalent bonds increase
357-403: The description of fibrinogen and fibrin, Alexander Schmidt hypothesised the existence of an enzyme that converts fibrinogen into fibrin in 1872. Prothrombin was discovered by Pekelharing in 1894. Thrombin is produced by the enzymatic cleavage of two sites on prothrombin by activated Factor X (Xa). The activity of factor Xa is greatly enhanced by binding to activated Factor V (Va), termed
378-406: The heavy chain into the β-barrel promoting the correct conformation of the catalytic residues. Contrary to crystal structures of active thrombin, hydrogen-deuterium exchange mass spectrometry studies indicate that this N-terminal Ile-NH3 does not become inserted into the β-barrel in the apo form of thrombin. However, binding of the active fragment of thrombomodulin appears to allosterically promote
399-641: The most common is factor V Leiden). The gene may be inherited heterozygous (1 pair), or much more rarely, homozygous (2 pairs), and is not related to gender or blood type. Homozygous mutations increase the risk of thrombosis more than heterozygous mutations, but the relative increased risk is not well documented. Other potential risks for thrombosis , such as oral contraceptives may be additive. The previously reported relationship of inflammatory bowel disease (i.e., Crohn's disease or ulcerative colitis ) and prothrombin G20210A or factor V Leiden mutation have been contradicted by research. Activation of prothrombin
420-424: The production of gamma-carboxyglutamic acid residues, slowing the activation of the coagulation cascade. In human adults, the normal blood level of antithrombin activity has been measured to be around 1.1 units/mL. Newborn levels of thrombin steadily increase after birth to reach normal adult levels, from a level of around 0.5 units/mL 1 day after birth, to a level of around 0.9 units/mL after 6 months of life. In
441-406: The stability of the fibrin clot. Thrombin interacts with thrombomodulin . As part of its activity in the coagulation cascade, thrombin also promotes platelet activation and aggregation via activation of protease-activated receptors on the cell membrane of the platelet. Thrombin bound to thrombomodulin activates protein C , an inhibitor of the coagulation cascade. The activation of protein C
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