151-530: 5–8: Tara Valoczki The Milton Hershey School , formerly the Hershey Industrial School , is a private boarding school in Hershey , Pennsylvania for K–12 students. The institution was founded in 1909 by chocolate industrialist Milton Hershey and his wife, Catherine Hershey . The school began with four students in 1910. Initially for only white male orphans, the school expanded in
302-493: A long terminal repeat (LTR). Regions in the LTR act as switches to control production of new viruses and can be triggered by proteins from either HIV or the host cell. The Psi element is involved in viral genome packaging and recognized by gag and rev proteins. The SLIP element ( TTTTTT ) is involved in the frameshift in the gag - pol reading frame required to make functional pol . The term viral tropism refers to
453-663: A Japanese school and a German school. Chinese schools were being founded by the ethnic Chinese in Malaysia as early as the 19th century. They provide secondary education in the Chinese language as the continuation of the primary education in Chinese national-type primary schools. The main medium of instruction in these schools is Mandarin Chinese using simplified Chinese characters . Being private schools, Chinese independent high schools do not receive consistent funding from
604-412: A board of governors and have a system of governance that ensures their independent operation. Private schools retain the right to select their students and are funded in whole or in part by charging their students for tuition , rather than relying on taxation through public (government) funding; at some private schools students may be eligible for a scholarship, lowering this tuition fee, dependent on
755-535: A cell by a virion can be called "cell-free spread" to distinguish it from a more recently recognized process called "cell-to-cell spread". In cell-free spread (see figure), virus particles bud from an infected T cell, enter the blood or extracellular fluid and then infect another T cell following a chance encounter. HIV can also disseminate by direct transmission from one cell to another by a process of cell-to-cell spread, for which two pathways have been described. Firstly, an infected T cell can transmit virus directly to
906-481: A cell surface. The unusual processing and high density means that almost all broadly neutralising antibodies that have so far been identified (from a subset of patients that have been infected for many months to years) bind to, or are adapted to cope with, these envelope glycans. The molecular structure of the viral spike has now been determined by X-ray crystallography and cryogenic electron microscopy . These advances in structural biology were made possible due to
1057-709: A certain minimum education; Irish state subsidised fee-charging schools must still work towards the Junior Certificate and the Leaving Certificate , for example. The single fee-charging secondary school in Ireland which receives no state funding, the Nord Anglia International School Dublin , does not have to undergo the state supervision which all the other fee-charging schools undergo. Students there also sit
1208-705: A condition in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Without treatment, the average survival time after infection with HIV is estimated to be 9 to 11 years, depending on the HIV subtype . In most cases, HIV is a sexually transmitted infection and occurs by contact with or transfer of blood , pre-ejaculate , semen , and vaginal fluids . Non-sexual transmission can occur from an infected mother to her infant during pregnancy , during childbirth by exposure to her blood or vaginal fluid, and through breast milk . Within these bodily fluids, HIV
1359-413: A fee-charging school wishes to employ extra teachers they are paid for with school fees, which tend to be relatively low in Ireland compared to the rest of the world. Because state funding plays a fundamental role in the finances of all but one fee-charging school, they must undergo similar state inspection to non-fee-charging schools. This is due to the requirement that the state ensure that children receive
1510-414: A few tested specimens might provide inconclusive results because of a low quantity specimen. In these situations, a second specimen is collected and tested for HIV infection. Modern HIV testing is extremely accurate, when the window period is taken into consideration. A single screening test is correct more than 99% of the time. The chance of a false-positive result in a standard two-step testing protocol
1661-451: A human host cell when the newly formed virus particle buds from the cell. The viral envelope contains proteins from the host cell and relatively few copies of the HIV envelope protein, which consists of a cap made of three molecules known as glycoprotein (gp) 120 , and a stem consisting of three gp41 molecules that anchor the structure into the viral envelope. The envelope protein, encoded by
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#17327827263781812-574: A key step in the progression to AIDS. A number of studies with subtype B-infected individuals have determined that between 40 and 50 percent of AIDS patients can harbour viruses of the SI and, it is presumed, the X4 phenotypes. HIV-2 is much less pathogenic than HIV-1 and is restricted in its worldwide distribution to West Africa . The adoption of "accessory genes" by HIV-2 and its more promiscuous pattern of co-receptor usage (including CD4-independence) may assist
1963-403: A more accurate distinction is between fee-charging schools and non-fee-charging schools. This is because approximately 85% of all schools are private schools ( Irish : scoil phríobháideach ) by virtue of not being owned by the state. The Roman Catholic Church is the largest owner of schools in Ireland, with other religious institutions owning the remaining private schools. Nevertheless, despite
2114-613: A more stable conformation following the NC binding, in which both the DIS and the U5:AUG regions of the gRNA participate in extensive base pairing. RNA can also be processed to produce mature messenger RNAs (mRNAs). In most cases, this processing involves RNA splicing to produce mRNAs that are shorter than the full-length genome. Which part of the RNA is removed during RNA splicing determines which of
2265-447: A number of mechanisms, including pyroptosis of abortively infected T cells, apoptosis of uninfected bystander cells, direct viral killing of infected cells, and killing of infected CD4 T cells by CD8 cytotoxic lymphocytes that recognize infected cells. When CD4 T cell numbers decline below a critical level, cell-mediated immunity is lost, and the body becomes progressively more susceptible to opportunistic infections, leading to
2416-450: A permanent trust fund that would address the needs of the private education sector in the country. In Portugal, private schools were traditionally set up by foreign expatriates and diplomats in order to cater for their educational needs. Portuguese-speaking private schools are widespread across Portugal's main cities. International private schools are mainly concentrated in and around Lisbon , Porto , Braga , Coimbra and Covilhã , across
2567-907: A public school refers to membership of the Headmasters' and Headmistresses' Conference , and this includes many independent grammar schools . The term 'public school' historically meant that the school was open to the public (as opposed to private tutors or the school being in private ownership). Many private schools actively compete with prestigious state schools (including state grammar schools and single sex schools ) in academic achievements. There are many old, world-renowned institutions in England that served as inspiration for most schools of their type abroad. These schools include Winchester College , Eton College , St Paul's School , Harrow School , Westminster School , and Rugby School . The Independent Schools Inspectorate regularly publishes reports on
2718-469: A religious affiliation, the precise use of the term excludes parochial (and other) schools if there is a financial dependence upon, or governance subordinate to, outside organizations. These definitions generally apply equally to both primary and secondary education . In the United Kingdom and several other Commonwealth countries including Australia, Canada and New Zealand the use of the term
2869-511: A report by Geeta Gandhi Kingdon entitled: The Emptying of Public Schools and Growth of Private Schools in India , it is said that for sensible education-policy making, it is vital to take account of the various changing trends in the size of the private and public schooling sectors in India. Ignoring these trends involves the risk of poor policies/legislation, with adverse effects on children's education. A lot of criticism towards Iranian government
3020-447: A revenue of US$ 450 million in 2008, but is projected to be a US$ 40 billion market. Although there are private schools in India, they are highly regulated in terms of what they can teach, in what form they can operate (must be a non-profit to run any accredited educational institution) and all the other aspects of the operation. Hence, the differentiation between government schools and private schools can be misleading. However, in
3171-461: A science high school. The Fund for Assistance to Private Education (FAPE) is a perpetual trust fund for private education created by Executive Order № 156 s. 1968 and amended by Executive Order № 150 s. 1994. FAPE was created on November 5, 1968 by Executive Order No. 156, in implementation of the project agreement between the Philippine and United States governments to establish
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#17327827263783322-512: A state school, e.g. they must teach the state curriculum, they must employ registered teachers, and they can not charge tuition fees (they can charge "attendance dues" for the upkeep on the still-private school land and buildings). The largest decline in private school numbers occurred between 1979 and 1984, when the nation's then-private Catholic school system integrated. As a result, private schools in New Zealand are now largely restricted to
3473-464: A strain of SIV found in sooty mangabees. Since HIV-1 is derived from SIVcpz, and HIV-2 from SIVsm, the genetic sequence of HIV-2 is only partially homologous to HIV-1 and more closely resembles that of SIVsm. Many HIV-positive people are unaware that they are infected with the virus. For example, in 2001 less than 1% of the sexually active urban population in Africa had been tested, and this proportion
3624-497: A student's talents or abilities (e.g., sports scholarship, art scholarship, academic scholarship), need for financial aid, or tax credit scholarships that might be available. Roughly one in 10 U.S. families have chosen to enroll their children in private school for the past century. Some private schools are associated with a particular religious denomination or religion , such as Roman Catholicism , various branches of Protestantism or Judaism . Although private schools may have
3775-558: A target T cell via a virological synapse . Secondly, an antigen-presenting cell (APC), such as a macrophage or dendritic cell, can transmit HIV to T cells by a process that either involves productive infection (in the case of macrophages) or capture and transfer of virions in trans (in the case of dendritic cells). Whichever pathway is used, infection by cell-to-cell transfer is reported to be much more efficient than cell-free virus spread. A number of factors contribute to this increased efficiency, including polarised virus budding towards
3926-423: A trust for the school, valued at $ 60 million altogether. The school grew quickly, with about 60 boys in 1915, 352 boys in 1931, and 1034 boys in 1937. Non-sectarian Christian religious training was mandatory. In 1933, the age range of admitted orphans was expanded from 4–8 years to 4–14. In 1934, a junior-senior high school building was opened at the site with a capacity for 1500 students. Hershey resigned from
4077-659: Is The King’s School , founded in 1831. Catholic schools form the second-largest sector after government schools, with around 21% of secondary enrolments. Catholic schools, typically have a strong religious focus and usually most of the staff and students are Catholic. In 1999, 5.6% of Canadian students were enrolled in private schools, some of which are religious or faith-based schools, including Christian, Jewish, and Islamic schools. Some private schools in Canada are considered world-class, especially some boarding schools. Private schools have sometimes been controversial, with some in
4228-460: Is an adaptation for repair of genome damage, and that recombinational variation is a byproduct that may provide a separate benefit. The final step of the viral cycle, assembly of new HIV-1 virions, begins at the plasma membrane of the host cell. The Env polyprotein (gp160) goes through the endoplasmic reticulum and is transported to the Golgi apparatus where it is cleaved by furin resulting in
4379-476: Is any for which the facilities and funding are not provided by the federal, state or local government, as opposed to a public school, which is operated by the government - or in the case of charter schools , independently with government funding and regulation. A majority of private schools in the United States are operated by religious institutions and organizations. In practice, the term "private school"
4530-412: Is because of large gap of University entrance Exam success between public and private school students, number private school growth has seen a 15% growth dominating Iranian education sector. there is a rise of "luxury" schools. Studying for one year term in private schools may cost 50 million as of July 2023. In Ireland, the internationally recognised definition of "private school" is misleading and
4681-516: Is bound with the CD4 protein, the envelope complex undergoes a structural change, exposing the chemokine receptor binding domains of gp120 and allowing them to interact with the target chemokine receptor. This allows for a more stable two-pronged attachment, which allows the N-terminal fusion peptide gp41 to penetrate the cell membrane. Repeat sequences in gp41, HR1, and HR2 then interact, causing
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4832-477: Is commonly used to denote a non-sectarian school, whereas "church school", "Christian school", and (for Catholic institutions) "parochial school" are used to denote sectarian ones. Independent schools in the United States educate a tiny fraction of the school-age population (slightly over 1% of the entire school-age population). The essential distinction between independent schools and other private schools
4983-424: Is even lower in rural populations. Furthermore, in 2001 only 0.5% of pregnant women attending urban health facilities were counselled, tested or received their test results. Again, this proportion is even lower in rural health facilities. Since donors may therefore be unaware of their infection, donor blood and blood products used in medicine and medical research are routinely screened for HIV. HIV-1 testing
5134-818: Is generally free, schools that offer instruction in languages other than the official languages of Finland (for example in English) may charge modest fees. There are also unlicensed private schools that do not follow any national curriculum and do not receive public funding. Certificates obtained from such institutions are not recognized as valid proof of education. In 2018, private schools accounted for 3% of basic education institutions and 9% of upper secondary education institutions. All Universities of Applied Sciences are either privately owned or municipality-owned companies or foundations. Some private Universities in Finland operate without legal mandate and are not overseen by
5285-737: Is generally restricted to primary and secondary educational levels, and it is almost never used of universities or other tertiary institutions. Private education in North America covers the whole gamut of educational activity, ranging from pre-school to tertiary level institutions. Annual tuition fees at K–12 schools range from nothing at so called 'tuition-free' schools to more than $ 45,000 at several New England University-preparatory schools . The secondary level includes university-preparatory schools, boarding schools , and day schools . Tuition at private secondary schools varies from school to school and depends on many factors, including
5436-464: Is high as the glycans shield the underlying viral protein from neutralisation by antibodies. This is one of the most densely glycosylated molecules known and the density is sufficiently high to prevent the normal maturation process of glycans during biogenesis in the endoplasmic and Golgi apparatus. The majority of the glycans are therefore stalled as immature 'high-mannose' glycans not normally present on human glycoproteins that are secreted or present on
5587-399: Is initially done using an enzyme-linked immunosorbent assay (ELISA) to detect antibodies to HIV-1. Specimens with a non-reactive result from the initial ELISA are considered HIV-negative, unless new exposure to an infected partner or partner of unknown HIV status has occurred. Specimens with a reactive ELISA result are retested in duplicate. If the result of either duplicate test is reactive,
5738-447: Is largely confined to West Africa . HIV is similar in structure to other retroviruses. It is roughly spherical with a diameter of about 120 nm , around 100,000 times smaller in volume than a red blood cell . It is composed of two copies of positive- sense single-stranded RNA that codes for the virus' nine genes enclosed by a conical capsid composed of 2,000 copies of the viral protein p24 . The single-stranded RNA
5889-549: Is more likely, leading to immunodeficiency. Three groups of HIV-1 have been identified on the basis of differences in the envelope ( env ) region: M, N, and O. Group M is the most prevalent and is subdivided into eight subtypes (or clades ), based on the whole genome, which are geographically distinct. The most prevalent are subtypes B (found mainly in North America and Europe), A and D (found mainly in Africa), and C (found mainly in Africa and Asia); these subtypes form branches in
6040-420: Is officially non-sectarian. The school is free for students and is funded by a trust containing most of Hershey's fortune, valued at about US$ 15 billion, making it the wealthiest U.S. private school. Nearly half of the trust's money comes from its controlling interest in Hershey's eponymous chocolate company . Milton S. Hershey , creator of The Hershey Company , was a chocolate industrialist and had founded
6191-479: Is on a sliding scale and still has a "capacity to pay" element; however, on average, funding granted to the private school sector is 40 percent of that required to operate government schools, the remainder being made up by tuition fees and donations from parents. The majority of the funding comes from the Commonwealth Government, while the state and territory governments provide about one-third of
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6342-561: Is present as both free virus particles and virus within infected immune cells . Research has shown (for both same-sex and opposite-sex couples) that HIV is not contagious during sexual intercourse without a condom if the HIV-positive partner has a consistently undetectable viral load . HIV infects vital cells in the human immune system, such as helper T cells (specifically CD4 T cells), macrophages , and dendritic cells . HIV infection leads to low levels of CD4 T cells through
6493-564: Is self-governance and financial independence. In contrast, public schools are funded and governed by local and state governments, and most parochial schools are owned, governed, and financed by religious institutions such as a diocese or parish. Independent schools may be affiliated with a particular religion or denomination; however, unlike parochial schools, independent schools are self-owned and governed by independent boards of trustees. While independent schools are not subject to significant government oversight or regulation, they are accredited by
6644-653: Is the unique characteristic of this segment of the education industry, offering schools four freedoms that contribute to their success: the freedom to define their own unique missions; the freedom to admit and keep only those students well-matched to the mission; the freedom to define the qualifications for high quality teachers; and the freedom to determine on their own what to teach and how to assess student achievement and progress. HIV The human immunodeficiency viruses ( HIV ) are two species of Lentivirus (a subgroup of retrovirus ) that infect humans. Over time, they cause acquired immunodeficiency syndrome (AIDS),
6795-617: Is their decision whether to engage tutors". Some of the oldest schools in South Africa are private church schools that were established by missionaries in the early nineteenth century. The private sector has grown ever since. After the abolition of apartheid, the laws governing private education in South Africa changed significantly. The South African Schools Act of 1996 recognizes two categories of schools: "public" (state-controlled) and "independent" (which includes traditional private schools and schools which are privately governed). In
6946-412: Is tightly bound to nucleocapsid proteins, p7, and enzymes needed for the development of the virion such as reverse transcriptase , proteases , ribonuclease and integrase . A matrix composed of the viral protein p17 surrounds the capsid ensuring the integrity of the virion particle. This is, in turn, surrounded by the viral envelope , that is composed of the lipid bilayer taken from the membrane of
7097-534: Is unknown how often such mixed packaging occurs under natural conditions. Bonhoeffer et al. suggested that template switching by reverse transcriptase acts as a repair process to deal with breaks in the single-stranded RNA genome. In addition, Hu and Temin suggested that recombination is an adaptation for repair of damage in the RNA genomes. Strand switching (copy-choice recombination) by reverse transcriptase could generate an undamaged copy of genomic DNA from two damaged single-stranded RNA genome copies. This view of
7248-530: The Shin Min Daily News estimated that there were around 540 tuition centres offering private tuition in Singapore. Due to their high demand, tuition centres are able to charge high fees for their services; they have an annual turnover of SGD$ 110.6 million in 2005. The official government stance on private tuition is that "it understands parents want the best for their children and that it
7399-638: The African green monkey (SIVagm) and sooty mangabey (SIVsmm) are thought to have a long evolutionary history with their hosts. These hosts have adapted to the presence of the virus, which is present at high levels in the host's blood, but evokes only a mild immune response, does not cause the development of simian AIDS, and does not undergo the extensive mutation and recombination typical of HIV infection in humans. In contrast, when these strains infect species that have not adapted to SIV ("heterologous" or similar hosts such as rhesus or cynomologus macaques ),
7550-544: The CCR5-Δ32 mutation are resistant to infection by the R5 virus, as the mutation leaves HIV unable to bind to this co-receptor, reducing its ability to infect target cells. Sexual intercourse is the major mode of HIV transmission. Both X4 and R5 HIV are present in the seminal fluid , which enables the virus to be transmitted from a male to his sexual partner . The virions can then infect numerous cellular targets and disseminate into
7701-601: The International Baccalaureate rather than the Irish Leaving Certificate which every other Irish secondary school student sits. In exchange, however, Nord Anglia students pay some €25,000 per annum in fees, compared to c. €4,000 – €8,000 per annum fees by students in all other fee-charging Irish schools. Many fee-charging schools in Ireland also double as boarding schools. The fees for these may then rise up to €25,000 per year. All
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#17327827263787852-589: The Presidential Award for Excellence in Mathematics and Science Teaching . Official website [REDACTED] Private school A private school is a school not administered or funded by the government , unlike a public school . Private schools (also known as 'independent schools') are schools that are not dependent upon national or local government to finance their financial endowment . Unless privately owned they typically have
8003-727: The Scottish Council of Independent Schools (SCIS). All schools are still inspected by the state inspectorate, Education Scotland , and the Care Inspectorate . Independent schools in Scotland that are charities are subject to a specific test from the Office of the Scottish Charity Regulator , designed to demonstrate the public benefit the schools provide. In the United States, a private school
8154-418: The U.S. Supreme Court , which ordered the racial desegregation of Girard College , Milton Hershey School admitted its first non-white student. School official James E. Bobb, however, stated that the decision to admit racial minorities was unrelated to the ruling. In 1976, the school expanded its definition of orphanhood to include "social orphans", those with single or divorced parents. In November of that year,
8305-445: The adsorption of glycoproteins on its surface to receptors on the target cell followed by fusion of the viral envelope with the target cell membrane and the release of the HIV capsid into the cell. Entry to the cell begins through interaction of the trimeric envelope complex ( gp160 spike) on the HIV viral envelope and both CD4 and a chemokine co-receptor (generally either CCR5 or CXCR4 , but others are known to interact) on
8456-421: The gag polyproteins still need to be cleaved into the actual matrix, capsid and nucleocapsid proteins. This cleavage is mediated by the packaged viral protease and can be inhibited by antiretroviral drugs of the protease inhibitor class. The various structural components then assemble to produce a mature HIV virion. Only mature virions are then able to infect another cell. The classical process of infection of
8607-514: The microtubule -based transport to the nucleus, the viral single-strand RNA genome is transcribed into double-strand DNA, which is then integrated into a host chromosome. HIV can infect dendritic cells (DCs) by this CD4-CCR5 route, but another route using mannose-specific C-type lectin receptors such as DC-SIGN can also be used. DCs are one of the first cells encountered by the virus during sexual transmission. They are currently thought to play an important role by transmitting HIV to T cells when
8758-403: The phylogenetic tree representing the lineage of the M group of HIV-1. Co-infection with distinct subtypes gives rise to circulating recombinant forms (CRFs). In 2000, the last year in which an analysis of global subtype prevalence was made, 47.2% of infections worldwide were of subtype C, 26.7% were of subtype A/CRF02_AG, 12.3% were of subtype B, 5.3% were of subtype D, 3.2% were of CRF_AE, and
8909-438: The α -chemokine receptor, CXCR4 , for entry. Dual-tropic HIV-1 strains are thought to be transitional strains of HIV-1 and thus are able to use both CCR5 and CXCR4 as co-receptors for viral entry. The α -chemokine SDF-1 , a ligand for CXCR4, suppresses replication of T-tropic HIV-1 isolates. It does this by down-regulating the expression of CXCR4 on the surface of HIV target cells. M-tropic HIV-1 isolates that use only
9060-427: The β -chemokine receptor, CCR5 , for entry and are thus able to replicate in both macrophages and CD4 T cells. This CCR5 co-receptor is used by almost all primary HIV-1 isolates regardless of viral genetic subtype. Indeed, macrophages play a key role in several critical aspects of HIV infection. They appear to be the first cells infected by HIV and perhaps the source of HIV production when CD4 cells become depleted in
9211-605: The (first) Gonski Report , the funding formula was changed to compute individual school funding compared to a School Resourcing Standard (SRS). The SRS uses exam results from the National Assessment Program – Literacy and Numeracy tests, calculates the SRS from a cohort of well-performing schools, and applies this formula to other schools on the assumption that they should be able to achieve similar results from similar funding. The funding provided to private schools
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#17327827263789362-431: The 1960s and 70s to include girls, racial minorities, and "social orphans"—those with impoverished parents. About 2,000 students attended the school in 2020. Admission is restricted to low-income individuals aged 4–15 without intellectual or behavioral problems. Students live in group homes of uniform sex and similar age, with set schedules for elementary, middle, and high school students. The school has Christian elements but
9513-587: The CCR5 receptor are termed R5; those that use only CXCR4 are termed X4, and those that use both, X4R5. However, the use of co-receptors alone does not explain viral tropism, as not all R5 viruses are able to use CCR5 on macrophages for a productive infection and HIV can also infect a subtype of myeloid dendritic cells , which probably constitute a reservoir that maintains infection when CD4 T cell numbers have declined to extremely low levels. Some people are resistant to certain strains of HIV. For example, people with
9664-726: The Catholic schismatic group , the Society of St Pius X in Wanganui operates three private schools (including the secondary school, St Dominic's College ). A recent group of private schools run as a business has been formed by Academic Colleges Group ; with schools throughout Auckland, including ACG Senior College in Auckland's CBD, ACG Parnell College in Parnell , and international school ACG New Zealand International College . In
9815-532: The Commonwealth amount. The Turnbull government commissioned Gonski in 2017 to chair the independent Review to Achieve Educational Excellence in Australian Schools, commonly called Gonski 2.0. The government published the report on 30 April 2018. Following negotiation, bilateral agreements between the Commonwealth of Australia with each state and territory commenced on 1 January 2019, with
9966-405: The DIS (dimerization initiation signal) hairpin is exposed. The formation of the gRNA dimer is mediated by a 'kissing' interaction between the DIS hairpin loops of the gRNA monomers. At the same time, certain guanosine residues in the gRNA are made available for binding of the nucleocapsid (NC) protein leading to the subsequent virion assembly. The labile gRNA dimer has been also reported to achieve
10117-419: The HIV env gene, allows the virus to attach to target cells and fuse the viral envelope with the target cell's membrane releasing the viral contents into the cell and initiating the infectious cycle. As the sole viral protein on the surface of the virus, the envelope protein is a major target for HIV vaccine efforts. Over half of the mass of the trimeric envelope spike is N-linked glycans . The density
10268-441: The HIV protein-coding sequences is translated. Mature HIV mRNAs are exported from the nucleus into the cytoplasm , where they are translated to produce HIV proteins, including Rev . As the newly produced Rev protein is produced it moves to the nucleus, where it binds to full-length, unspliced copies of virus RNAs and allows them to leave the nucleus. Some of these full-length RNAs function as mRNAs that are translated to produce
10419-570: The HIV virological synapse in vivo . The many dissemination mechanisms available to HIV contribute to the virus' ongoing replication in spite of anti-retroviral therapies. HIV differs from many viruses in that it has very high genetic variability . This diversity is a result of its fast replication cycle , with the generation of about 10 virions every day, coupled with a high mutation rate of approximately 3 x 10 per nucleotide base per cycle of replication and recombinogenic properties of reverse transcriptase. This complex scenario leads to
10570-526: The LTR promoter acting by binding the TAR RNA element. The TAR may also be processed into microRNAs that regulate the apoptosis genes ERCC1 and IER3 . The rev protein (p19) is involved in shuttling RNAs from the nucleus and the cytoplasm by binding to the RRE RNA element. The vif protein (p23) prevents the action of APOBEC3G (a cellular protein that deaminates cytidine to uridine in
10721-545: The Malaysian government, although they did receive some funding from some state governments as well as in the 2019 and 2020 budgets under the Pakatan Harapan government. However, in accordance with their aim of providing affordable education to all in the Chinese language, their school fees are substantially lower than those of most other private schools. The schools are kept alive almost exclusively by donations from
10872-625: The Ministry of Education and Culture. Graduates from these universities must undergo the same verification process as those holding foreign degrees. The right to create private schools in Germany is in Article 7, Paragraph 4 of the Grundgesetz and cannot be suspended even in a state of emergency . It is also not possible to abolish these rights. This unusual protection of private schools
11023-612: The Philippines, the private sector has been a major provider of educational services. Private schools are generally free to determine their curriculum in accordance with existing laws and regulations. Science high schools are special schools for the more intellectually promising students to foster the problem-solving approach of critical thinking. As separate high schools, they have specific characteristics not found in regular high schools. However, any private or public high school can aspire to meet these minimum standards and be considered
11174-665: The Portuguese region of Algarve , and in the autonomous region of Madeira . The Ministério da Educação acts as the supervisory and regulatory body for all schools, including international schools. In Singapore, after Primary School Leaving Examination (PSLE), students can choose to enter a private high school. Private tuition is a lucrative industry in Singapore, since many parents send their children for private tuition after school. A straw poll by The Straits Times newspaper in 2008 found that out of 100 students interviewed, only 3 students did not have any form of tuition. In 2010,
11325-433: The U.S.: Although IFA can be used to confirm infection in these ambiguous cases, this assay is not widely used. In general, a second specimen should be collected more than a month later and retested for persons with indeterminate western blot results. Although much less commonly available, nucleic acid testing (e.g., viral RNA or proviral DNA amplification method) can also help diagnosis in certain situations. In addition,
11476-409: The adaptive benefit of recombination in HIV could explain why each HIV particle contains two complete genomes, rather than one. Furthermore, the view that recombination is a repair process implies that the benefit of repair can occur at each replication cycle, and that this benefit can be realized whether or not the two genomes differ genetically. On the view that recombination in HIV is a repair process,
11627-565: The adoption of the Right to Education (RTE) Act 2009, private schools were required to be 'government-recognised'. A private school would be eligible for government recognition when it met certain conditions. At the primary and secondary level, India has a large private school system complementing the government run schools, with 29% of students receiving private education in the 6 to 14 age group. Certain post-secondary technical schools are also private. The private education market in India had
11778-423: The advent of AIDS. HIV-positive patients acquire an enormously broad spectrum of opportunistic infections, which was particularly problematic prior to the onset of HAART therapies; however, the same infections are reported among HIV-infected patients examined post-mortem following the onset of antiretroviral therapies. Thus, during the course of infection, viral adaptation to the use of CXCR4 instead of CCR5 may be
11929-403: The animals develop AIDS and the virus generates genetic diversity similar to what is seen in human HIV infection. Chimpanzee SIV (SIVcpz), the closest genetic relative of HIV-1, is associated with increased mortality and AIDS-like symptoms in its natural host. SIVcpz appears to have been transmitted relatively recently to chimpanzee and human populations, so their hosts have not yet adapted to
12080-472: The boy's family and a reversal of the policy. In 2013, 14-year-old student Abbie Bartels committed suicide shortly after being denied attendance at the school's eighth-grade graduation. While the family brought a lawsuit against the school, the U.S. Court of Appeals for the 3rd Circuit refused in early 2021 to revive the lawsuit, agreeing that the case was tragic but that the school could not be held responsible for her death. In 2016, eleven former students sued
12231-592: The cell as new virus particles that will begin the replication cycle anew. Two types of HIV have been characterized: HIV-1 and HIV-2. HIV-1 is the virus that was initially discovered and termed both lymphadenopathy associated virus (LAV) and human T-lymphotropic virus 3 (HTLV-III). HIV-1 is more virulent and more infective than HIV-2, and is the cause of the majority of HIV infections globally. The lower infectivity of HIV-2, compared to HIV-1, implies that fewer of those exposed to HIV-2 will be infected per exposure. Due to its relatively poor capacity for transmission, HIV-2
12382-470: The cell types a virus infects. HIV can infect a variety of immune cells such as CD4 T cells , macrophages , and microglial cells . HIV-1 entry to macrophages and CD4 T cells is mediated through interaction of the virion envelope glycoproteins (gp120) with the CD4 molecule on the target cells' membrane and also with chemokine co-receptors . Macrophage-tropic (M-tropic) strains of HIV-1, or non- syncytia -inducing strains (NSI; now called R5 viruses ) use
12533-459: The collapse of the extracellular portion of gp41 into a hairpin shape. This loop structure brings the virus and cell membranes close together, allowing fusion of the membranes and subsequent entry of the viral capsid. After HIV has bound to the target cell, the HIV RNA and various enzymes, including reverse transcriptase, integrase, ribonuclease, and protease, are injected into the cell. During
12684-497: The college level as first-generation college students. Other similar partnerships are with Mansfield University , Shippensburg University , East Stroudsburg University , Indiana University of Pennsylvania and Millersville University . In 2018, they signed a similar agreement with Dickinson College . The school is split into three cohorts: pre-K through 4th grade, 5th through 8th grade, and 9th through 12th grade. As of 2020, there are 180 student homes, each with 8 to 12 students of
12835-447: The development of AIDS. HIV is a member of the genus Lentivirus , part of the family Retroviridae . Lentiviruses have many morphologies and biological properties in common. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period . Lentiviruses are transmitted as single-stranded , positive- sense , enveloped RNA viruses . Upon entry into
12986-577: The development of stable recombinant forms of the viral spike by the introduction of an intersubunit disulphide bond and an isoleucine to proline mutation ( radical replacement of an amino acid) in gp41. The so-called SOSIP trimers not only reproduce the antigenic properties of the native viral spike, but also display the same degree of immature glycans as presented on the native virus. Recombinant trimeric viral spikes are promising vaccine candidates as they display less non-neutralising epitopes than recombinant monomeric gp120, which act to suppress
13137-507: The development of up to six cost-free early childhood resource centers in Pennsylvania. Named Catherine Hershey Schools for Early Learning, the first center opened in 2023, and they serve children from birth to age five from economically disadvantaged and at-risk backgrounds. In 2017, Milton Hershey School created a partnership with Penn State University , its seventh such partnership to offer graduates of Milton Hershey School support at
13288-650: The education system has always been separate from the rest of Great Britain , the term 'public school' was used historically to refer to state schools for the general public. According to The Good Schools Guide about 7% of children being educated in the United Kingdom are at fee-charging schools at GCSE level and 13% at A-level. Some independent schools are single-sex, although this is becoming less common. In 2011, fees range from under £3,000 to £21,000 and above per year for day pupils, rising to over £27,000 per year for boarders. Costs differ in Scotland. One in four independently educated children come from postcodes with
13439-613: The eight campuses, the main campus is by far the largest at 3,340 acres. In 2016, Milton Hershey School's science curriculum supervisor Dr. Jaunine Fouche received the Presidential Award for Excellence in Mathematics and Science Teaching . In 2017, the Law, Public Safety and Security program at Milton Hershey School was recognized as the Advance CTE National Program of Excellence. In 2019, Milton Hershey School's elementary innovation lab instructor Joel Crowley received
13590-401: The entire student population. Private school numbers have been in decline since the mid-1970s as a result of many private schools opting to become state-integrated schools , mostly due to financial difficulties stemming from changes in student numbers or the economy. State-integrated schools keep their private school special character and receives state funds in return for having to operate like
13741-449: The exception of Victoria, whose bilateral agreement commenced on 1 February 2019. The funding agreements provide states with funding for government schools (20 percent) and non-government schools (80 percent) taking into consideration annual changes in enrolment numbers, indexation and student or school characteristics. A National School Resourcing Board was charged with the responsibility of independently reviewing each state's compliance with
13892-473: The expense of enrolments in government schools, which have seen their enrolment share reduce from 78.1 percent to 65 percent since 1970, although the rate of growth of private schools has slowed in the later years. Australian private schools differ from those in other OECD nations as the Australian Government provides funding to all schools including private schools. In 2013, after release of
14043-528: The final years of the apartheid era, parents at white government schools were given the option to convert to a "semi-private" form called Model C , and many of these schools changed their admissions policies to accept children classified to be of other races. These schools tend to produce better academic results than government schools formerly reserved for other "race groups". Former "Model C" schools are state-controlled, not private. All schools in South Africa (including both independent and public schools) have
14194-760: The funding agreement(s). Private school fees can vary from under $ 100 per month to $ 2,000 and upwards, depending on the student's year level, the school's size, and the socio-economics of the school community. In late 2018 it was reported the most expensive private schools (such as AAGPS and CAS schools in New South Wales , GPS and QGSSSA schools in Queensland , AGSV and APS schools in Victoria ) charge fees of up to $ 500,000 for thirteen years of education. The oldest private school in Australia
14345-431: The generation of many variants of HIV in a single infected patient in the course of one day. This variability is compounded when a single cell is simultaneously infected by two or more different strains of HIV. When simultaneous infection occurs, the genome of progeny virions may be composed of RNA strands from two different strains. This hybrid virion then infects a new cell where it undergoes replication. As this happens,
14496-576: The generation of recombinational variation would be a consequence, but not the cause of, the evolution of template switching. HIV-1 infection causes chronic inflammation and production of reactive oxygen species . Thus, the HIV genome may be vulnerable to oxidative damage , including breaks in the single-stranded RNA. For HIV, as well as for viruses in general, successful infection depends on overcoming host defense strategies that often include production of genome-damaging reactive oxygen species. Thus, Michod et al. suggested that recombination by viruses
14647-552: The genetic information that is transmitted from parental to progeny genomes. Viral recombination produces genetic variation that likely contributes to the evolution of resistance to anti-retroviral therapy . Recombination may also contribute, in principle, to overcoming the immune defenses of the host. Yet, for the adaptive advantages of genetic variation to be realized, the two viral genomes packaged in individual infecting virus particles need to have arisen from separate progenitor parental viruses of differing genetic constitution. It
14798-495: The government, it can be an aided or an unaided school. Demand for private schools has been growing over the years. While a consensus over what is the most significant driver of this growth in private schooling has not yet emerged, some authors have attributed this to a higher demand for English-medium education, a dissatisfaction with the quality of public schools, greater affordability of private schools, and non-availability of preferred field of study in government schools. After
14949-415: The immune response to target epitopes. The RNA genome consists of at least seven structural landmarks ( LTR , TAR , RRE , PE, SLIP, CRS, and INS), and nine genes ( gag , pol , and env , tat , rev , nef , vif , vpr , vpu , and sometimes a tenth tev , which is a fusion of tat , env and rev ), encoding 19 proteins. Three of these genes, gag , pol , and env , contain information needed to make
15100-521: The integrated DNA provirus is transcribed into RNA. The full-length genomic RNAs (gRNA) can be packaged into new viral particles in a pseudodiploid form. The selectivity in the packaging is explained by the structural properties of the dimeric conformer of the gRNA. The gRNA dimer is characterized by a tandem three-way junction within the gRNA monomer, in which the SD and AUG hairpins , responsible for splicing and translation respectively, are sequestered and
15251-1362: The largest cities (Auckland, Hamilton, Wellington and Christchurch) and niche markets. Private schools are almost fully funded by tuition fees paid by students' parents, but they do receive some government subsidies. Private schools are popular for academic and sporting performance, prestige, exclusivity and old boys/girls networks; however, many state-integrated schools and some prestigious single-sex state schools, such as Auckland Grammar School and Wellington College , are actively competitive with private schools in academic and sporting achievement, history and character. Private schools are often Anglican, such as King's College and Diocesan School for Girls in Auckland, St Paul's Collegiate School in Hamilton, St Peter's School in Cambridge , Samuel Marsden Collegiate School in Wellington, and Christ's College and St Margaret's College in Christchurch; or Presbyterian, such as Saint Kentigern College and St Cuthbert's College in Auckland, Scots College and Queen Margaret College in Wellington, and St Andrew's College and Rangi Ruru Girls' School in Christchurch. However,
15402-602: The lawsuit in 2020. In 2017, Milton Hershey School received its first accreditation from the Commission on Accreditation for Law Enforcement Agencies (CALEA) for its campus security. In 2020, Milton Hershey School received its second CALEA accreditation recognizing the school's Central Monitoring staff for excellence in Public Safety Communications. Milton Hershey School's endowment was worth around $ 15 billion in 2020, about half of which comes from
15553-575: The learning of disabled children. In Australia, private schools are mostly operated by an independently elected school council or board of governors and range broadly in the type of school-education provided and the socio-economics of the school community served. Most private schools are run by religious institutes while others have no religious affiliation and are driven by a national philosophy (such as international schools ), pedogogical philosophy (such as Waldorf-Steiner schools ), or specific needs (such as special schools ). Australia has one of
15704-647: The low spending was because the school "can't seem to conceivably find any way to spend this pile of money". School officials countered that the school must adhere to the spending limits of Hershey's original deed. In July 2002, the company managing the school trust announced it was considering selling its share in the Hershey company. Alumni and residents criticized the idea; some accused the trust of long-term financial wrongdoing and incompetence, with 1980 graduate Ric Fouad calling it "the charitable-trust Enron ". In 2020, Milton Hershey School invested $ 350 million to fund
15855-524: The media and in Ontario's Provincial Ministry of Education asserting that students may buy inflated grades from private schools. In Finland, education takes place in state-run, municipality-run, and private schools. To qualify for public funding, all schools must receive a license from the Ministry of Education and Culture and align with the national curriculum and educational standards. While education
16006-570: The most privatised education systems in the world with 30 per cent of primary students and more than 40 per cent of secondary students attending private schools. In contrast the OECD average is 18 per cent. Catholic schools make up a sizeable proportion of total enrolment (nearly 15%) and are usually regarded as a school sector of their own within the broad category of private schools, often charging lower fees than Protestant private schools. Enrolment in non-government schools have been growing steadily at
16157-429: The name of educational quality, most comply with regulations relating to the educational content of classes. Religious private schools often add religious instruction to the courses provided by local public schools. Special assistance schools aim to improve the lives of their students by providing services tailored to the particular needs of individual students. Such schools include tutoring schools and schools to assist
16308-433: The national average income or below, and one in three receive assistance with school fees. Evidence from a major longitudinal study suggests that British independent schools provide advantages in educational attainment and access to top universities. In England and Wales, the more prestigious independent schools are known as ' public schools ', sometimes subdivided into major and minor public schools. A modern definition of
16459-491: The next two decades, the school grew to about 2,000 students. In 2010, the school settled in a molestation case involving sixteen children. In late 2011, a 13 year old was denied admission to the school because he was HIV -positive. While the school initially defended its decision, citing safety concerns, an anti-discrimination lawsuit filed by the AIDS Law Project of Pennsylvania led to a settlement of $ 700,000 for
16610-609: The opportunity to choose the school they prefer. For instance, the biggest school chain, Kunskapsskolan ("The Knowledge School"), offers 30 schools and a web-based environment, has 700 employees and teaches nearly 10,000 pupils. Private schools are also known as independent schools, because of their freedom to operate outside government and local government control. Elite institutions for older pupils, which charge high fees, are typically described as public schools . Preparatory schools in England and Wales prepare pupils up to 13 years old to enter public schools. In Scotland, where
16761-410: The patient. Macrophages and microglial cells are the cells infected by HIV in the central nervous system . In the tonsils and adenoids of HIV-infected patients, macrophages fuse into multinucleated giant cells that produce huge amounts of virus. T-tropic strains of HIV-1, or syncytia -inducing strains (SI; now called X4 viruses ) replicate in primary CD4 T cells as well as in macrophages and use
16912-589: The public. In the Netherlands over two-thirds of state-funded schools operate autonomously, with many of these schools being linked to faith groups. The Programme for International Student Assessment , coordinated by the OECD , ranks the education in the Netherlands as the 9th best in the world as of 2008, being significantly higher than the OECD average. As of April 2014, there were 88 private schools in New Zealand, catering for around 28,000 students or 3.7% of
17063-442: The quality of education in all independent schools. In Scotland, schools not state -funded are known as independent or private schools. Independent schools may also be specialist or special schools – such as some music schools, Steiner Waldorf Education schools, or special education schools. Scottish independent schools currently educate over 31,000 students and employ approximately 3,500 teachers. Schools are represented by
17214-406: The remaining 5.3% were composed of other subtypes and CRFs. Most HIV-1 research is focused on subtype B; few laboratories focus on the other subtypes. The existence of a fourth group, "P", has been hypothesised based on a virus isolated in 2009. The strain is apparently derived from gorilla SIV (SIVgor), first isolated from western lowland gorillas in 2006. HIV-2's closest relative is SIVsm,
17365-425: The reverse transcriptase, by jumping back and forth between the two different RNA templates, will generate a newly synthesized retroviral DNA sequence that is a recombinant between the two parental genomes. This recombination is most obvious when it occurs between subtypes. The closely related simian immunodeficiency virus (SIV) has evolved into many strains, classified by the natural host species. SIV strains of
17516-437: The right to set compulsory school fees, and formerly model C schools tend to set much higher school fees than other public schools. In Sweden, pupils are free to choose a private school and the private school gets paid the same amount as municipal schools. Over 10% of Swedish pupils were enrolled in private schools in 2008. Sweden is internationally known for this innovative school voucher model that provides Swedish pupils with
17667-485: The same gender and similar age, and each led by house parents. Upon entering 5th grade, students begin the Career and Technical Education program, which provides activities tailored to students' specialized interests. The school offers college scholarships for some students and has special partnerships with about twenty colleges for student transitions. 85 to 90 percent of students continue to post-secondary education. In 2024,
17818-404: The same six regional accreditation agencies that accredit public schools . The National Association of Independent Schools (NAIS) is a membership organization of American pre-college independent schools. The NAIS provides this definition of an independent school: Independent schools are 501(c)(3) nonprofit corporate entities, independent in governance and finance, meaning: Independence
17969-424: The school for invasion of privacy, alleging that an employee had hidden a camera in a bathroom for senior male students. The employee admitted to filming the boys' showering and was sentenced to a year in prison. In 2017, former student Adam Dobson sued the school after the school expelled Dobson for attempting suicide. Dobson later stated that he was forced to watch a religious gay-conversion video. A court dismissed
18120-422: The school introduced ‘theme homes’ where high school students of similar interests live together and engage in experiential learning about potential career options. According to the school's website, students are admitted on five criteria: having a family income less than twice the federal poverty level , being age 4 to 15, having an IQ over 80, lacking serious behavioral problems, and being able to "benefit from
18271-551: The school successfully petitioned the Dauphin County Court on allowing girls based on their charter. The first eight girls arrived in March 1977. Female admission was gradual, first restricted to those between kindergarten and fifth grade, then in the summer of 1977 to sixth through eighth grade, and finally to all grade divisions in the summer of 1978. In October 1977, approximately 1100 boys and 60 girls were enrolled in
18422-412: The school's beginning. School guidelines prohibit pressuring students to convert faiths; some students, however, have reported being proselytized by their house parents. The school's address is 1201 Homestead Lane, Hershey , Pennsylvania , just off U.S. Highway 322 . As of 2015, the school trust owns 10,174 acres (4,117 ha) of land, about 7,500 acres (3,000 ha) of which are school campuses. Of
18573-401: The school's board in 1944, a year before his death. By then the school had acquired about 10,000 acres of land. The school's name was changed from Hershey Industrial School to Milton Hershey School in 1953, reportedly to eliminate the possibility of "industrial" connoting a reform school . The school's selection of students broadened in the 1960s and 70s. Following a 1968 decision upheld by
18724-553: The school's controlling share of The Hershey Company 's stock; the school is the US's wealthiest private school (besides universities and colleges). Only about 1.5% of this sum is used per year for educational programs, spending about $ 118,400 per year on each student. The proportionally low spending has been controversial. Massachusetts Institute of Technology professor John Core called it "ludicrous", and Georgetown University law professor Brian Galle called it "indefensibly low". Galle said
18875-470: The school's location, the willingness of parents to pay, peer tuitions, and the school's financial endowment . Some private schools are boarding schools, and many military academies are privately owned or operated as well. Religiously affiliated and denominational schools form a subcategory of private schools. Some such schools teach religious education , together with the usual academic subjects, to impress their particular faith's beliefs and traditions in
19026-412: The school's longtime tradition of requiring all grade 9–12 students to milk cows, twice daily, was rescinded, with strong approval from students and the board. Director of secondary education John Storm justified the change, stating that "the school revolved around the milking program, when in fact we wanted it to revolve around educational opportunities". In 2002, the school had about 1,500 students; over
19177-669: The school's program". The school is cost-free for students. As of 2021, 73 percent of students are from the state of Pennsylvania. The acceptance rate is low, around 14% in 1991. Each year, an average of 12.5% of the student population leaves, mostly by graduating or being removed by their parents. A quarter to a third of departures are expulsions. The school housed 2,020 students in the 2017–18 school year. Of these, 44.5% were white, 33.8% were black, 8.7% were Hispanic, 1.0% were Asian, and 11.0% identified with two or more races. There were 395 students in pre-K through 4th grade, 545 students in grades 5–8, and 950 students in grades 10–12. According to
19328-596: The school's website, there are roughly an equal number of boys and girls. While in 2000 only about one tenth of students had a deceased parent, nearly 90 percent of the school's come from single-parent homes as of 2020, with an average income of $ 22,000. The Compass Project enables Milton Hershey School students to develop leadership skills and build character. The school's Multicultural and Global Education program allows high school students to travel internationally and gain worldly experience and global awareness. Non-sectarian Christian religious training has been mandatory since
19479-532: The school; by September 1978, female students made up 10% of the 1300 students. Cyndi Jacobsen wrote in 1989 in The Sentinel that "students chafe under the rules, the lack of privacy and individuality, and the anachronistic dairy barns ... [b]ut graduates tend to look back at their experience as a survival test". She said that house parents varied widely in their treatment, with some "rigid and authoritarian" and others "warm and affirming." Later that year,
19630-519: The single-stranded viral DNA and/or interferes with reverse transcription ). The vpr protein (p14) arrests cell division at G2/M . The nef protein (p27) down-regulates CD4 (the major viral receptor), as well as the MHC class I and class II molecules. Nef also interacts with SH3 domains . The vpu protein (p16) influences the release of new virus particles from infected cells. The ends of each strand of HIV RNA contain an RNA sequence called
19781-412: The site of cell-to-cell contact, close apposition of cells, which minimizes fluid-phase diffusion of virions, and clustering of HIV entry receptors on the target cell towards the contact zone. Cell-to-cell spread is thought to be particularly important in lymphoid tissues , where CD4 T cells are densely packed and likely to interact frequently. Intravital imaging studies have supported the concept of
19932-673: The specimen is reported as repeatedly reactive and undergoes confirmatory testing with a more specific supplemental test (e.g., a polymerase chain reaction (PCR), western blot or, less commonly, an immunofluorescence assay (IFA)). Only specimens that are repeatedly reactive by ELISA and positive by IFA or PCR or reactive by western blot are considered HIV-positive and indicative of HIV infection. Specimens that are repeatedly ELISA-reactive occasionally provide an indeterminate western blot result, which may be either an incomplete antibody response to HIV in an infected person or nonspecific reactions in an uninfected person. HIV deaths in 2014 excluding
20083-406: The state-subsidised fee-charging schools are run by a religious order, e.g., the Society of Jesus or Congregation of Christian Brothers , etc. The major private schools being Blackrock College , Clongowes Wood College , Castleknock College , Belvedere College , Gonzaga College and Terenure College . There are also a few fee-charging international schools in Ireland, including a French school,
20234-556: The structural proteins Gag and Env. Gag proteins bind to copies of the virus RNA genome to package them into new virus particles. HIV-1 and HIV-2 appear to package their RNA differently. HIV-1 will bind to any appropriate RNA. HIV-2 will preferentially bind to the mRNA that was used to create the Gag protein itself. Two RNA genomes are encapsidated in each HIV-1 particle (see Structure and genome of HIV ). Upon infection and replication catalyzed by reverse transcriptase, recombination between
20385-496: The structural proteins for new virus particles. For example, env codes for a protein called gp160 that is cut in two by a cellular protease to form gp120 and gp41. The six remaining genes, tat , rev , nef , vif , vpr , and vpu (or vpx in the case of HIV-2), are regulatory genes for proteins that control the ability of HIV to infect cells, produce new copies of virus (replicate), or cause disease. The two tat proteins (p16 and p14) are transcriptional transactivators for
20536-641: The students who attend. Others use the denomination as a general label to describe what the founders based their belief, while still maintaining a fine distinction between academics and religion. They include parochial schools , a term which is often used to denote Roman Catholic schools. Other religious groups represented in the K–12 private education sector include Protestants, Jews, Muslims, and Orthodox Christians. Many educational alternatives , such as private schools or online schools , are privately financed. Private schools often avoid some state regulations, although in
20687-427: The target cell surface. Gp120 binds to integrin α 4 β 7 activating LFA-1 , the central integrin involved in the establishment of virological synapses , which facilitate efficient cell-to-cell spreading of HIV-1. The gp160 spike contains binding domains for both CD4 and chemokine receptors. The first step in fusion involves the high-affinity attachment of the CD4 binding domains of gp120 to CD4. Once gp120
20838-413: The target cell, the viral RNA genome is converted (reverse transcribed) into double-stranded DNA by a virally encoded enzyme, reverse transcriptase , that is transported along with the viral genome in the virus particle. The resulting viral DNA is then imported into the cell nucleus and integrated into the cellular DNA by a virally encoded enzyme, integrase , and host co-factors . Once integrated,
20989-659: The town of Hershey , Pennsylvania. On November 15, 1909, he and his wife, Catherine Hershey , signed over a 486-acre (1.97 km) piece of farmland, forming the Hershey Industrial School. The school accepted "poor, healthy, white male orphans" between the ages of four and eight, allowing them to stay until the age of eighteen. The first four boys were admitted in September 1910. In 1918, following Catherine's death three years prior, Milton put most of his fortune—including his share of his company's stock—into
21140-414: The two HIV envelope glycoproteins, gp41 and gp120 . These are transported to the plasma membrane of the host cell where gp41 anchors gp120 to the membrane of the infected cell. The Gag (p55) and Gag-Pol (p160) polyproteins also associate with the inner surface of the plasma membrane along with the HIV genomic RNA as the forming virion begins to bud from the host cell. The budded virion is still immature as
21291-484: The two genomes can occur. Recombination occurs as the single-strand, positive-sense RNA genomes are reverse transcribed to form DNA. During reverse transcription, the nascent DNA can switch multiple times between the two copies of the viral RNA. This form of recombination is known as copy-choice. Recombination events may occur throughout the genome. Anywhere from two to 20 recombination events per genome may occur at each replication cycle, and these events can rapidly shuffle
21442-471: The vast majority of schools being under the ownership of private institutions, a large majority of all their costs, including teachers' salaries, are paid for by the Irish state. Of these private schools, only a very small minority actually charge fees. In 2007, 'The number of schools permitted to charge fees represents 7.6% of the 723 post primary level schools and they cater for 7.1% of the total enrolment.' If
21593-570: The viral DNA into the host cell's genome is carried out by another viral enzyme called integrase . The integrated viral DNA may then lie dormant, in the latent stage of HIV infection. To actively produce the virus, certain cellular transcription factors need to be present, the most important of which is NF- κ B (nuclear factor kappa B), which is upregulated when T cells become activated. This means that those cells most likely to be targeted, entered and subsequently killed by HIV are those actively fighting infection. During viral replication,
21744-536: The virus in its adaptation to avoid innate restriction factors present in host cells. Adaptation to use normal cellular machinery to enable transmission and productive infection has also aided the establishment of HIV-2 replication in humans. A survival strategy for any infectious agent is not to kill its host, but ultimately become a commensal organism. Having achieved a low pathogenicity, over time, variants that are more successful at transmission will be selected. The HIV virion enters macrophages and CD4 T cells by
21895-425: The virus is captured in the mucosa by DCs. The presence of FEZ-1 , which occurs naturally in neurons , is believed to prevent the infection of cells by HIV. HIV-1 entry, as well as entry of many other retroviruses, has long been believed to occur exclusively at the plasma membrane. More recently, however, productive infection by pH -independent, clathrin-mediated endocytosis of HIV-1 has also been reported and
22046-463: The virus may become latent , allowing the virus and its host cell to avoid detection by the immune system, for an indeterminate amount of time. The virus can remain dormant in the human body for up to ten years after primary infection; during this period the virus does not cause symptoms. Alternatively, the integrated viral DNA may be transcribed , producing new RNA genomes and viral proteins, using host cell resources, that are packaged and released from
22197-403: The virus to evade the body's immune system. The reverse transcriptase also has ribonuclease activity that degrades the viral RNA during the synthesis of cDNA, as well as DNA-dependent DNA polymerase activity that creates a sense DNA from the antisense cDNA. Together, the cDNA and its complement form a double-stranded viral DNA that is then transported into the cell nucleus . The integration of
22348-540: The virus. This virus has also lost a function of the nef gene that is present in most SIVs. For non-pathogenic SIV variants, nef suppresses T cell activation through the CD3 marker. Nef 's function in non-pathogenic forms of SIV is to downregulate expression of inflammatory cytokines , MHC-1 , and signals that affect T cell trafficking. In HIV-1 and SIVcpz, nef does not inhibit T-cell activation and it has lost this function. Without this function, T cell depletion
22499-475: The whole organism. However, a selection process leads to a predominant transmission of the R5 virus through this pathway. In patients infected with subtype B HIV-1, there is often a co-receptor switch in late-stage disease and T-tropic variants that can infect a variety of T cells through CXCR4. These variants then replicate more aggressively with heightened virulence that causes rapid T cell depletion, immune system collapse, and opportunistic infections that mark
22650-477: Was implemented to protect these schools from a second Gleichschaltung or similar event in the future. In Italy education is predominantly public; about one-fifth of schools are private, attended by about one out of 10 Italian schoolchildren. The Italian constitution states that education is to be public, free, and compulsory for at least eight years. In India, private schools are called independent schools, but since some private schools receive financial aid from
22801-448: Was recently suggested to constitute the only route of productive entry. Shortly after the viral capsid enters the cell, an enzyme called reverse transcriptase liberates the positive-sense single-stranded RNA genome from the attached viral proteins and copies it into a complementary DNA (cDNA) molecule. The process of reverse transcription is extremely error-prone, and the resulting mutations may cause drug resistance or allow
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